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dtx3l protein expression level  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc dtx3l protein expression level
    Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L <t>(DTX3L).</t> ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).
    Dtx3l Protein Expression Level, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtx3l protein expression level/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    dtx3l protein expression level - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats"

    Article Title: Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

    Journal: Biomolecules

    doi: 10.3390/biom11030472

    Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).
    Figure Legend Snippet: Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).

    Techniques Used: Ubiquitin Proteomics, Binding Assay, Luciferase, Sequencing, Mutagenesis, Transfection, Negative Control, Reporter Assay, Activity Assay

    Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.
    Figure Legend Snippet: Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.

    Techniques Used: Immunohistochemical staining, Staining, Negative Control



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    GraphPad Software Inc dtx3l protein expression level
    Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L <t>(DTX3L).</t> ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).
    Dtx3l Protein Expression Level, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtx3l protein expression level/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    dtx3l protein expression level - by Bioz Stars, 2026-05
    90/100 stars
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    Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).

    Journal: Biomolecules

    Article Title: Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

    doi: 10.3390/biom11030472

    Figure Lengend Snippet: Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).

    Article Snippet: Differences in wildtype (WT) or mutant (Mut) DTX3L 3′UTR luciferase reports and the expression level of DTX3L protein under two conditions were compared using Student’s t -test (GraphPad Prism version 8.0, San Diego, CA, USA).

    Techniques: Ubiquitin Proteomics, Binding Assay, Luciferase, Sequencing, Mutagenesis, Transfection, Negative Control, Reporter Assay, Activity Assay

    Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.

    Journal: Biomolecules

    Article Title: Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

    doi: 10.3390/biom11030472

    Figure Lengend Snippet: Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.

    Article Snippet: Differences in wildtype (WT) or mutant (Mut) DTX3L 3′UTR luciferase reports and the expression level of DTX3L protein under two conditions were compared using Student’s t -test (GraphPad Prism version 8.0, San Diego, CA, USA).

    Techniques: Immunohistochemical staining, Staining, Negative Control